Journal: bioRxiv

Article Title: Investigating the coupled effects of stiffness and stretch on the trabecular meshwork cells using a hydrogel-integrated microfluidic system

doi: 10.64898/2026.04.17.717863

Figure Lengend Snippet: (A) Schematic illustration and a photograph of the hydrogel-integrated microfluidic system showing the multi-layer architecture. (B) Cross-sectional view demonstrating hydraulic pressure application through the microfluidic channel to induce equi-biaxial stretch on the integrated hydrogel.

Article Snippet: The inflatable microfluidic chip was designed in AutoCAD (Autodesk, USA) and fabricated on microscope slides (Fisherbrand 12-550-A3, Fisher Scientific, USA) using five PDMS-based layers ( ): (1) hydraulic pressure channel layer, (2) PDMS membrane layer, (3) hydrogel channel layer, (4) well layer, and (5) port layer.

Techniques:

Journal: bioRxiv

Article Title: Investigating the coupled effects of stiffness and stretch on the trabecular meshwork cells using a hydrogel-integrated microfluidic system

doi: 10.64898/2026.04.17.717863

Figure Lengend Snippet: The PDMS surface was treated with oxygen plasma to form a hydroxyl group (OH-PDMS). 10% TMSPMA was treated to form a methacrylate functional group on the surface (TMSPMA-PDMS). A Sigmacote-coated coverslip was placed on the TMSPMA-PDMS to form an instant microfluidic channel. A patterned Rubylith film was placed on the bottom of the microfluidic system. GelMA hydrogel was introduced from the center inlet to form four hydrogels simultaneously. UV was exposed from the bottom of the chip. Coverslips were removed to create a flat hydrogel surface.

Article Snippet: The inflatable microfluidic chip was designed in AutoCAD (Autodesk, USA) and fabricated on microscope slides (Fisherbrand 12-550-A3, Fisher Scientific, USA) using five PDMS-based layers ( ): (1) hydraulic pressure channel layer, (2) PDMS membrane layer, (3) hydrogel channel layer, (4) well layer, and (5) port layer.

Techniques: Clinical Proteomics, Functional Assay

Journal: Bioactive Materials

Article Title: Harnessing bi-exosome combination alleviates osteoarthritis progression

doi: 10.1016/j.bioactmat.2025.11.050

Figure Lengend Snippet: Evolution of the in vivo anti-osteoarthritic effects of Exo. (A) Anti-osteoarthritic scheme timeline: (i) Fabrication of Exo-loaded microspheres using microfluidic technology; (ii) The OA rats were indued by joint destabilization and over-erosion for 4 weeks followed by two intraarticular injections with 2 weeks interval. Measurements were conducted at the 2 weeks and 12 weeks post-2nd drug administration; (iii) Mechanical sensitivity (Von Frey) test; (iv) Mobility (Gait) analysis. Figure was created using BioRender. (B) Representative morphology of the microsphere in oil phase, scale bar: 50 μm. (C) Representative image showing DiI labeled B‐Exo and DiO labeled C‐Exo inside microsphere, scale bar: 50 μm. White dash circles indicate the microsphere outer boundaries. (D) Size distribution analysis of Exo-loaded microspheres. (E) Release curve of protein-containing Exo from Exo-loaded microspheres in vitro . (F) The pain threshold of the hind paw in rats at 12-week. (G) Quantification analysis of stride length, stand time, swing time, and swing speed of rats at 12-week. (H and J) Representative μCT images of osteophyte after 12-week joint injection, and quantification analysis of the osteophyte volume, scale bar: 5 mm. (I) The width of knee joint in rats at 12-week. (K) The changes of synovial fluid cytokine (TNF-α, IL-6, and IL-1β) levels in 12-week rat knee joints. Data are given as mean ± SD, n = 4 rats per group (except for (E), n = 3). Statistical significance was determined by one-way ANOVA and Tukey's multiple comparisons test.

Article Snippet: These two phases were mixed to form droplets at the junction within the microfluidic chip flow channel (Regenovo, DY01-1661).

Techniques: In Vivo, Labeling, In Vitro, Injection